Molecular and cellular neuroscience, 2003, Vol.22 (1), p.107-117
2003
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- Autor(en) / Beteiligte
- Titel
- Activity blockade increases the number of functional synapses in the hippocampus of newborn rats
- Ist Teil von
- Molecular and cellular neuroscience, 2003, Vol.22 (1), p.107-117
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2003
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- During development neuronal circuitries are refined by activity. Here we studied the role of spontaneous electrical activity in the regulation of synapse formation in the intact newborn (Postnatal Day 3; P3) rat hippocampus in vitro. The blockade of the spontaneous network activity with TTX led to an increase in the number of functional excitatory synapses in the CA3 area of the developing hippocampus. In parallel, there was a substantial increase in the expression levels of the presynaptic markers synaptophysin, synaptotagmin, and synapsin I and of GluR1 AMPA receptor subunits. These changes were associated with an increase in the frequency and amplitude of AMPA receptor-mediated miniature excitatory postsynaptic currents (mEPSCs). Our correlated immunocytochemical, electronmicroscopical, and electrophysiological experiments indicate that in the developing hippocampus spontaneous network activity controls the number of functional synapses.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1044-7431eISSN: 1095-9327DOI: 10.1016/S1044-7431(02)00012-XTitel-ID: cdi_proquest_miscellaneous_18685401
- Format
- –
- Schlagworte
- Action Potentials - drug effects, Action Potentials - physiology, Animals, Animals, Newborn, Calcium-Binding Proteins, Cell Differentiation - drug effects, Cell Differentiation - physiology, Dendrites - metabolism, Dendrites - ultrastructure, Excitatory Amino Acid Antagonists - pharmacology, Excitatory Postsynaptic Potentials - drug effects, Excitatory Postsynaptic Potentials - physiology, Hippocampus - growth & development, Hippocampus - metabolism, Hippocampus - ultrastructure, Immunohistochemistry, Membrane Glycoproteins - metabolism, Microscopy, Electron, Nerve Tissue Proteins - metabolism, Neural Pathways - growth & development, Neural Pathways - metabolism, Neural Pathways - ultrastructure, Neuronal Plasticity - drug effects, Neuronal Plasticity - physiology, Organ Culture Techniques, Presynaptic Terminals - drug effects, Presynaptic Terminals - metabolism, Presynaptic Terminals - ultrastructure, Rats, Rats, Wistar, Receptors, AMPA - metabolism, Sodium Channel Blockers - pharmacology, Synapsins - metabolism, Synaptic Membranes - metabolism, Synaptic Membranes - ultrastructure, Synaptic Transmission - drug effects, Synaptic Transmission - physiology, Synaptophysin - metabolism, Synaptotagmins, Tetrodotoxin - pharmacology