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TET3 Mediates Alterations in the Epigenetic Marker 5hmC and Akt pathway in Steroid‐Associated Osteonecrosis
Journal of bone and mineral research, 2017-02, Vol.32 (2), p.319-332
Zhao, Jie
Ma, Xin‐long
Ma, Jian‐xiong
Sun, Lei
Lu, Bin
Wang, Ying
Xing, Guo‐sheng
Wang, Yan
Dong, Ben‐chao
Xu, Li‐yan
Kuang, Ming‐Jie
Fu, Lin
Bai, Hao‐hao
Ma, Yue
Jin, Wei‐lin
2017
Volltextzugriff (PDF)
Details
Autor(en) / Beteiligte
Zhao, Jie
Ma, Xin‐long
Ma, Jian‐xiong
Sun, Lei
Lu, Bin
Wang, Ying
Xing, Guo‐sheng
Wang, Yan
Dong, Ben‐chao
Xu, Li‐yan
Kuang, Ming‐Jie
Fu, Lin
Bai, Hao‐hao
Ma, Yue
Jin, Wei‐lin
Titel
TET3 Mediates Alterations in the Epigenetic Marker 5hmC and Akt pathway in Steroid‐Associated Osteonecrosis
Ist Teil von
Journal of bone and mineral research, 2017-02, Vol.32 (2), p.319-332
Ort / Verlag
United States: Wiley Subscription Services, Inc
Erscheinungsjahr
2017
Quelle
MEDLINE
Beschreibungen/Notizen
ABSTRACT Steroid‐associated osteonecrosis (SAON) is one of the common complications of clinical glucocorticoid (GC) administration, with osteocyte apoptosis appearing as the primary histopathological lesion. However, the precise mechanism underlying SAON remains unknown. Epigenetic modification may be a major cause of SAON. Recently, cumulative research revealed that Ten‐Eleven Translocation (TET) proteins can catalyze the conversion of 5‐methylcytosine (5mC) to 5‐hydroxymethylcytosine (5hmC) and then alter the epigenetic state of DNA. Here, we report that TET3‐5hmC was upregulated in the femoral head tissues of SAON patients and MLO‐Y4 cells with dexamethasone (Dex) treatment. Knockdown of TET3 in MLO‐Y4 cells decreased 5hmC enrichment and rescued Dex‐induced apoptosis. Meanwhile, the local intramedullary injection of TET3 siRNA in Sprague‐Dawley rats abrogated GC‐induced osteocyte apoptosis, histopathological changes, abnormal MRI signals, and bone microstructure declines in the femoral head in vivo. Moreover, a hydroxymethylated DNA immunoprecipitation (hMeDIP)‐chip analysis of Dex‐treated osteocytes revealed 456 different 5hmC‐enriched genes. The Akt pathway was found to mediate the functional effect of Dex‐induced dynamic 5hmC change; this was further verified in clinical samples. The loss of TET3 in MLO‐Y4 cells abrogated Dex‐induced Akt signaling pathway inhibition. Therefore, our data for the first time identify the effect of TET3‐5hmC on the Akt pathway and the necessity of this signaling cascade in SAON, identifying a new potential therapeutic target. © 2016 American Society for Bone and Mineral Research.
Sprache
Englisch
Identifikatoren
ISSN: 0884-0431
eISSN: 1523-4681
DOI: 10.1002/jbmr.2992
Titel-ID: cdi_proquest_miscellaneous_1868315816
Format
–
Schlagworte
5-Methylcytosine - analogs & derivatives
,
5-Methylcytosine - metabolism
,
Aged
,
Aged, 80 and over
,
Animals
,
APOPTOSIS
,
Apoptosis - drug effects
,
Dexamethasone - pharmacology
,
Dioxygenases - metabolism
,
DNA DEMETHYLATION
,
DNA-Binding Proteins - metabolism
,
Epigenesis, Genetic - drug effects
,
EPIGENETICS
,
Femur Head - metabolism
,
Gene Knockdown Techniques
,
Humans
,
Mice
,
Middle Aged
,
Models, Biological
,
Osteocytes - pathology
,
OSTEONECROSIS
,
Osteonecrosis - chemically induced
,
Osteonecrosis - genetics
,
Proto-Oncogene Proteins - metabolism
,
Proto-Oncogene Proteins c-akt - metabolism
,
Signal Transduction - drug effects
,
Steroids - adverse effects
,
TET3
,
Up-Regulation
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