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Metabolic engineering of Escherichia coli to produce gamma-aminobutyric acid using xylose
Ist Teil von
Applied microbiology and biotechnology, 2017-05, Vol.101 (9), p.3587-3603
Ort / Verlag
Berlin/Heidelberg: Springer Berlin Heidelberg
Erscheinungsjahr
2017
Link zum Volltext
Quelle
2022 ECC(Springer)
Beschreibungen/Notizen
Biomass-derived xylose is an economically interesting substrate for the sustainable microbial production of value-added compounds.
Escherichia coli
could barely use xylose to directly produce gamma-aminobutyric acid. In this study,
E. coli
strains that could directly produce gamma-aminobutyric acid were developed through the deletion of eight genes
sucA
,
puuE
,
gabT
,
gabP
,
xylA
,
xylB
,
waaC
, and
waaF
, and the overexpression of two
E. coli
genes
gadB
and
gdhA
, as well as five
Caulobacter crescent
genes
CcxylA
,
CcxylB
,
CcxylC
,
CcxylD
, and
CcxylX
. Both
E. coli
strains W3110 and JM109 could directly produce gamma-aminobutyric acid from xylose after either overexpression of the seven genes or deletion of the eight genes. Overexpression of the seven genes of in the multiple deletion mutants further increased gamma-aminobutyric acid production. Among the 28 recombinant
E. coli
strains constructed in this study, the highest gamma-aminobutyric acid was produced by JWZ08/pWZt7-g3/pWZt7-xyl. JWZ08/pWZt7-g3/pWZt7-xyl could produce 3.95 g/L gamma-aminobutyric acid in flask cultivation, using xylose as the sole carbon source.