Details

Autor(en) / Beteiligte

• Small, Brian C

Titel

Anesthetic efficacy of metomidate and comparison of plasma cortisol responses to tricaine methanesulfonate, quinaldine and clove oil anesthetized channel catfish Ictalurus punctatus

Ist Teil von

• Aquaculture, 2003-03, Vol.218 (1), p.177-185

Ort / Verlag

Amsterdam: Elsevier B.V

Erscheinungsjahr

2003

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• The present experiments were designed to determine the efficacy of metomidate hydrochloride as an alternative anesthetic with potential cortisol blocking properties for channel catfish Ictalurus punctatus. Channel catfish (75 g) were exposed to concentrations of metomidate ranging from 0.5 to 16 ppm for a period of 60 min. At 16-ppm metomidate, mortality occurred in 65% of the catfish. No mortalities were observed at concentrations of 8 ppm or less. The minimum concentration of metomidate producing desirable anesthetic properties was 6 ppm. At this concentration, acceptable induction and recovery times were observed in catfish ranging from 3 to 810 g average body weight. Plasma cortisol levels during metomidate anesthesia (6 ppm) were compared to fish anesthetized with tricaine methanesulfonate (100 ppm), quinaldine (30 ppm) and clove oil (100 ppm). Cortisol levels of catfish treated with metomidate and clove oil remained at baseline levels during 30 min of anesthesia ( P>0.05). Plasma cortisol levels of tricaine methanesulfonate and quinaldine anesthetized catfish peaked approximately eight- and fourfold higher ( P<0.05), respectively, than fish treated with metomidate. These results suggest that the physiological disturbance of channel catfish during routine-handling procedures and stress-related research could be reduced through the use of metomidate as an anesthetic.