Details

Autor(en) / Beteiligte

• Martino, N.A.
• Marzano, G.
• Mangiacotti, M.
• Miedico, O.
• Sardanelli, A.M.
• Gnoni, A.
• Lacalandra, G.M.
• Chiaravalle, A.E.
• Ciani, E.
• Bogliolo, L.
• Minervini, F.
• Pizzi, F.
• Dell’Aquila, M.E.

Titel

Exposure to cadmium during in vitro maturation at environmental nanomolar levels impairs oocyte fertilization through oxidative damage: A large animal model study

Ist Teil von

• Reproductive toxicology (Elmsford, N.Y.), 2017-04, Vol.69, p.132-145

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2017

Quelle

Elsevier ScienceDirect Journals

Beschreibungen/Notizen

• •Cadmium bioaccumulation at nanomolar levels occurs in ovine ovaries.•Cadmium exposure at nanomolar levels during IVM impairs ovine oocyte fertilization.•Nanomolar cadmium during IVM induces cumulus-oocyte oxidative damage. Cadmium is a highly toxic heavy metal with negative effects on oocyte fertilization. The aim of this study was to analyse whether cadmium-induced impairment of fertilization is caused by mitochondria dysfunction and oxidative stress in the cumulus-oocyte complex (COC). Preliminarily, 19 trace element levels were measured in ovaries from juvenile and adult ewes and age-related cadmium ovarian bioaccumulation at nanomolar concentrations was found. COCs from juvenile and adult ewes, exposed during in vitro maturation to 1nM or 100nM CdCl2, and subjected to in vitro fertilization showed significantly lower fertilization rates in exposed COCs compared with controls. In vitro matured exposed and control COCs underwent confocal microscopy analysis of mitochondria activity and reactive oxygen species (ROS) levels and lipid peroxidation (LPO) assay at cumulus cell and oocyte level. In both age groups, cadmium at nanomolar concentrations induced cumulus-oocyte mitochondria over-activity and oxidative damage which were related to impaired oocyte fertilization.