Details

Autor(en) / Beteiligte

• Cederquist, Kristin B.
• Liu, Bing
• Grima, Megan R.
• Dalack, Peter J.
• Mahorn, Jordan T.

Titel

Laser-fabricated gold nanoparticles for lateral flow immunoassays

Ist Teil von

• Colloids and surfaces, B, Biointerfaces, 2017-01, Vol.149, p.351-357

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2017

Quelle

MEDLINE

Beschreibungen/Notizen

• [Display omitted] •Gold nanoparticles made by laser ablation are easily modified with antibodies.•Monoclonal and polyclonal antibodies adsorb with similar densities.•Antibody density on PLA-gold nanoparticles was 2× of that on gold nanoparticles made by chemical reduction.•More antibodies lead to increased sensitivity in a sandwich lateral flow immunoassay. Gold nanoparticles fabricated by pulsed laser ablation are an attractive alternative over those made by chemical reduction, as they offer a more reactive, chemically-bare surface. In this manuscript, we investigate the interactions of these nanoparticles with different classes of antibodies and quantify surface coverage via a fluorescence-based displacement assay. Saturation surface coverage of monoclonal antibodies was found to be almost 2× higher for particles made by pulsed laser ablation (∼28 antibodies/particle) as opposed to those made by chemical means (∼17 antibodies/particle). This higher coverage translated into ∼2× better immunoassay sensitivity, demonstrated by a sandwich lateral flow assay for human chorionic gonadotropin. This work highlights the advantages of these pulsed laser-fabricated materials and showcases their unique properties for colorimetric assay development.