Details

Autor(en) / Beteiligte

• Zhang, Wei
• Liu, Jun
• Shi, Haigang
• Yang, Kun
• Wang, Pingli
• Wang, Gexia
• Liu, Na
• Wang, Huaiyu
• Ji, Junhui
• Chu, Paul K.

Titel

Communication between nitric oxide synthase and positively-charged surface and bone formation promotion

Ist Teil von

• Colloids and surfaces, B, Biointerfaces, 2016-12, Vol.148, p.354-362

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2016

Quelle

MEDLINE

Beschreibungen/Notizen

• The communication between nitric oxide synthase (NOS) and bone implants is firstly employed as a guidance to create an osteo-inductive interface on bone implant. This study provides clear evidence that properly positively-charged surface promotes bone formation by mediating the direct communication with NOS. [Display omitted] •A surface with high surface potential is constructed on bone implant.•The high potential surface mediates BMSCs to express distinct NOS level.•The high potential surface can induce new bone growth.•An excessively high surface potential produces substantial immunomodulatory effects. Despite the effects on physiology of bone marrow mesenchymal stem cells (BMSCs) and bone tissue, biological signal communication between bone implants and them is seldom employed as a guidance to create an osteo-inductive interface. Herein, the positively-charged surface is constructed on bone implant from the perspective of mediation of nitric oxide synthase (NOS) expression to signal BMSCs osteo-differentiation. In vitro and in vivo results indicate that the proper surface potential on the positively-charged surface affects NOS to express a high level of inducible nitric oxide synthase (iNOS) in three NOS isoforms of the contacted BMSCs, upregulates their osteogenetic expression, and ultimately foster new bone growth. However, an excessively high surface potential produces substantial immunomodulatory effects thereby offsetting the aforementioned advantages. This study demonstrates that fine-tuning of the positively-charged surface and proper utilization of the communication between NOS and bone implants promote bone formation.