Details

Autor(en) / Beteiligte

• Liu, Tong
• Li, Ruliang
• Pan, Tao
• Liu, Dacai
• Petersen, Robert B
• Wong, Boon-Seng
• Gambetti, Pierluigi
• Sy, Man Sun

Titel

Intercellular Transfer of the Cellular Prion Protein

Ist Teil von

• The Journal of biological chemistry, 2002-12, Vol.277 (49), p.47671-47678

Ort / Verlag

United States: American Society for Biochemistry and Molecular Biology

Erscheinungsjahr

2002

Link zum Volltext

Quelle

Elektronische Zeitschriftenbibliothek (Open access)

Beschreibungen/Notizen

• The cellular prion protein (PrP C ) is a glycosylphosphatidylinositol (GPI)-anchored protein. We investigated whether PrP C can move from one cell to another cell in a cell model. Little PrP C transfer was detected when a PrP C expressing human neuroblastoma cell line was cultured with the human erythroleukemia cells IA lacking PrP C . Efficient transfer of PrP C was detected with the presence of phorbol 12-myristate 13-acetate, an activator of protein kinase C. Maximum PrP C transfer was observed when both donor and recipient cells were activated. Furthermore, PrP C transfer required the GPI anchor and direct cell to cell contact. However, intercellular protein transfer is not limited to PrP C , another GPI-anchored protein, CD90, also transfers from the donor cells to acceptor cells after cellular activation. Therefore, this transfer process is GPI-anchor and cellular activation dependent. These findings suggest that the intercellular transfer of GPI-anchored proteins is a regulated process, and may have implications for the pathogenesis of prion disease.