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A microfluidic device for characterizing nuclear deformations
Ist Teil von
Lab on a chip, 2017-02, Vol.17 (5), p.85-813
Ort / Verlag
England
Erscheinungsjahr
2017
Link zum Volltext
Quelle
MEDLINE
Beschreibungen/Notizen
Cell nuclei experience and respond to a wide range of forces, both
in vivo
and
in vitro
. In order to characterize the nuclear response to physical stress, we developed a microfluidic chip and used it to apply mechanical stress to live cells and measure their nuclear deformability. The device design is optimized for the detection of both nucleus and cytoplasm, which can then be conveniently quantified using a custom-written Matlab program. We measured nuclear sizes and strains of embryonic stem cells, for which we observed negative Poisson ratios in the nuclei. In addition, we were able to detect changes in the nuclear response after treatment with actin depolymerizing and chromatin decondensing agents. Finally, we showed that the device can be used for biologically relevant high-resolution confocal imaging of cells under compression. Thus, the device presented here allows for accurate physical phenotyping at high throughput and has the potential to be applied to a range of cell types.
A high-throughput microfluidic workflow allows rapid mechanical characterisation and phenotyping of the nuclear stress response in embryonic stem cells.