Details

Autor(en) / Beteiligte

• Ma, Ya
• Shen, Xiao-Lei
• Zeng, Qiang
• Wang, Hai-Shui
• Wang, Li-Shi

Titel

A multi-walled carbon nanotubes based molecularly imprinted polymers electrochemical sensor for the sensitive determination of HIV-p24

Ist Teil von

• Talanta (Oxford), 2017-03, Vol.164, p.121-127

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2017

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• To develop a rapid, simple and sensitive method for the determination of human immunodeficiency virus p24 (HIV-p24), a novel molecularly imprinted polymers (MIPs) electrochemical sensor was constructed on the surface of a multi-walled carbon nanotubes (MWCNTs) modified glassy carbon electrode (GCE) by surface polymerization using acrylamide (AAM) as functional monomer, N,N′-methylenebisacrylamide (MBA) as cross-linking agent and ammonium persulphate (APS) as initiator. Each modification step was carefully examined by cyclic voltammetry (CV), differential pulse voltammetry (DPV) and scanning electron microscope (SEM). The proposed MIPs electrochemical biosensor exhibited specific recognition to HIV-p24 and displayed a broad linear detection range from 1.0×10−4 to 2ngcm−3 with a low detection limit of 0.083pgcm−3 (S/N=3). This performance is superior to most HIV-p24 sensors based on other methods. Meanwhile, this sensor possessed of good selectivity, repeatability, reproducibility, stability and was successfully applied for the determination of HIV-p24 in real human serum samples, giving satisfactory results. The accuracy and reliability of the sensor is further confirmed by enzyme-linked immunosorbent assay (ELISA). [Display omitted] •A new method for HIV-p24 detection with the advantages of molecularly imprinted polymers and electrochemical sensor.•MWCNTs on the surface of electrode to improve the electrode response.•The sensor displays broader linear and lower detection limit compared with the existed methods.•The sensor shows acceptable stability, reproducibility and good accuracy in human serum.