Details

Autor(en) / Beteiligte

• Li, J
• Dai, A G
• Huang, C Y
• Hu, R C

Titel

Effect of Krüppel like zinc finger transcription factor 2 on γ-glutamylcysteine synthetase in bronchial epithelial cells of chronic obstructive pulmonary disease

Ist Teil von

• Zhong hua yi xue za zhi, 2017-01, Vol.97 (2), p.112-118

Ort / Verlag

China

Erscheinungsjahr

2017

Quelle

MEDLINE

Beschreibungen/Notizen

• To research the regulation effects of Krüppel like zinc finger transcription factor 2 (KLF2) on γ-glutamylcysteine synthetase (γ-GCS) in airway epithelial cells of chronic obstructive pulmonary disease (COPD). (1) Human specimen experiment: lung tissue of pulmonary lobectomy patients with lung cancer with or without COPD was collected from Department of Thoracic Surgery of Hunan Cancer Hospital from December 2008 to December 2009. The patients were divided into COPD group and control group without COPD. The levels of KLF2, γ-GCS mRNA and protein expression in lung tissues were measured by immunohistochemistry and in situ hybridization (ISH). Then, the correlation between KLF2 and γ-GCS mRNA and protein expression levels were analyzed, as well as the correlation between KLF2 or γ-GCS protein and smoking index, percentage of forced expiratory volume in one second to predicted value (FEV %), percentage of forced expiratory volume in one second to forced vital capacity (FVC/FEV ). (2) Animal experiment: the prima