Details

Autor(en) / Beteiligte

• Han, Xiaolong
• Song, Wenxia
• Liu, Guodong
• Li, Zhonghai
• Yang, Piao
• Qu, Yinbo

Titel

Improving cellulase productivity of Penicillium oxalicum RE-10 by repeated fed-batch fermentation strategy

Ist Teil von

• Bioresource technology, 2017-03, Vol.227, p.155-163

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2017

Quelle

Elsevier ScienceDirect Journals

Beschreibungen/Notizen

• •Plackett-Burman design was performed to screen important medium components.•The maximum FPA was 8.61U/mL after medium optimization by RSM.•Repeated fed-batch strategy improved cellulase productivity significantly.•Cellulases produced by different fermentation strategies had similar performances. Medium optimization and repeated fed-batch fermentation were performed to improve the cellulase productivity by P. oxalicum RE-10 in submerged fermentation. First, Plackett-Burman design (PBD) and central composite design (CCD) were used to optimize the medium for cellulase production. PBD demonstrated wheat bran and NaNO3 had significant influences on cellulase production. The CCD results showed the maximum filter paper activity (FPA) production of 8.61U/mL could be achieved in Erlenmeyer flasks. The maximal FPA reached 12.69U/mL by submerged batch fermentation in a 7.5-L stirred tank, 1.76-fold higher than that on the original medium. Then, the repeated fed-batch fermentation strategy was performed successfully for increasing the cellulase productivity from 105.75U/L/h in batch fermentation to 158.38U/L/h. The cellulase activity and the glucan conversion of delignined corn cob residue hydrolysis had no significant difference between the enzymes sampled from different cycles of the repeated fed-batch fermentation and that from batch culture.