Details

Autor(en) / Beteiligte

• Leclerc, Eric
• Kimura, Keiichi
• Shinohara, Marie
• Danoy, Mathieu
• Le Gall, Morgane
• Kido, Taketomo
• Miyajima, Atsushi
• Fujii, Teruo
• Sakai, Yasuyuki

Titel

Comparison of the transcriptomic profile of hepatic human induced pluripotent stem like cells cultured in plates and in a 3D microscale dynamic environment

Ist Teil von

• Genomics (San Diego, Calif.), 2017-01, Vol.109 (1), p.16-26

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2017

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• We have compared the transcriptomic profiles of human induced pluripotent stem cells after their differentiation in hepatocytes like cells in plates and microfluidic biochips. The biochips provided a 3D and dynamic support during the cell differentiation when compared to the 2D static cultures in plates. The microarray have demonstrated the up regulation of important pathway related to liver development and maturation during the culture in biochips. Furthermore, the results of the transcriptomic profile, coupled with immunostaining, and RTqPCR analysis have shown typical biomarkers illustrating the presence of responders of biliary like cells, hepatocytes like cells, and endothelial like cells. However, the overall tissue still presented characteristic of immature and foetal patterns. Nevertheless, the biochip culture provided a specific micro-environment in which a complex multicellular differentiation toward liver could be oriented. •We performed iPS hepatic like cells culture in a microfluidic biochip.•We compared the transcriptomic profiles from biochips and plated cultures.•We found higher hepatic differentiation patterns in biochips.•We measured higher functional liver like activities in biochips.•Although encouraging results, the biochip cultures remained a liver fœtal stage.