Details

Autor(en) / Beteiligte

• Jorgensen, Anders
• Kalliokoski, Otto
• Forsberg, Kristin
• Breitenstein, Katrine
• Weimann, Allan
• Henriksen, Trine
• Hau, Jann
• Wörtwein, Gitta
• Poulsen, Henrik Enghusen
• Jorgensen, Martin Balslev

Titel

A chronic increase of corticosterone age-dependently reduces systemic DNA damage from oxidation in rats

Ist Teil von

• Free radical biology & medicine, 2017-03, Vol.104, p.64-74

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2017

Quelle

MEDLINE

Beschreibungen/Notizen

• Stress and depression are associated with an acceleration of brain and bodily aging; effects which have been attributed to chronic elevations of glucocorticoids. We tested the hypothesis that a three week administration of stress-associated levels of corticosterone (CORT, the principal rodent glucocorticoid) would increase systemic and CNS DNA and RNA damage from oxidation; a phenomenon known to be centrally involved in the aging process. We also hypothesized that older individuals would be more sensitive to this effect and that the chronic CORT administration would exacerbate age-related memory decline. Young and old male Sprague-Dawley rats were non-invasively administered CORT by voluntary ingestion of nut paste containing either CORT (25mg/kg) or vehicle for a total of 22 days. CORT increased the 24h urinary excretion of the hormone to the levels previously observed after experimental psychological stress and caused a downregulation of the glucocorticoid receptor in the CA1 area of the hippocampus. Contrary to our hypothesis, 24h excretion of 8-oxodG/8-oxoGuo (markers of DNA/RNA damage from oxidation) was reduced in CORT-treated young animals, whereas old animals showed no significant differences. In old animals, CORT caused a borderline significant reduction of RNA oxidation in CNS, which was paralleled by a normalization of performance in an object location memory test. To our knowledge, this is the first demonstration that chronic stress-associated levels of CORT can reduce nucleic acid damage from oxidation. These findings contradict the notion of elevated CORT as a mediator of the accelerated aging observed in stress and depression. •CORT was administered non-invasively to yield stress-physiological levels.•CORT caused a reduced calorie intake and weight loss/stalling of weight gain.•CORT reduced systemic DNA/RNA damage from oxidation in young animals.•CORT improved spatial memory and tended to reduce CNS RNA damage in old animals.