Details

Autor(en) / Beteiligte

• Bazhenova, Ekaterina Y.
• Bazovkina, Daria V.
• Kulikova, Elizabeth A.
• Fursenko, Dariya V.
• Khotskin, Nikita V.
• Lichman, Daria V.
• Kulikov, Alexander V.

Titel

C1473G polymorphism in mouse tryptophan hydroxylase-2 gene in the regulation of the reaction to emotional stress

Ist Teil von

• Neuroscience letters, 2017-02, Vol.640, p.105-110

Ort / Verlag

Ireland: Elsevier B.V

Erscheinungsjahr

2017

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• •C1473G polymorphism in Tph2 gene regulates enzyme activity in mouse brain.•The stress-induced c-Fos mRNA rise is more intensive in the brain of B6-1473G mice.•The polymorphism modifies the effect of stress on 5-HT metabolism in the hypothalamus. Neurotransmitter serotonin (5-HT) is involved in the regulation of stress response. Tryptophan hydroxylase-2 (TPH2) is the key enzyme of serotonin (5-HT) synthesis in the brain. C1473G polymorphism in Tph2 gene is the main factor defining the enzyme activity in the brain of laboratory mice. The effect of interaction between C1473G polymorphism and 30min restriction stress on the behavior in the open field test, c-Fos gene expression and 5-HT metabolism in the brain in adult male of B6-1473C and B6-1473G congenic mouse lines with high and low TPH2 activity was investigated. A significant effect of genotype x stress interaction on c-Fos mRNA in the hypothalamus (F1,21=10.66, p<0.001) and midbrain (F1,21=9.18, p<0.01) was observed. The stress-induced rise of c-Fos mRNA in these structures is more intensive in B6-1473G than in B6-1473C mice. A marked effect of genotype x stress interaction on 5-HT level in the cortex (F1,18=9.38, p<0.01) and 5-HIAA/5-HT turnover rate in the hypothalamus (F1,18=9.01, p<0.01) was revealed. The restriction significantly decreased 5-HT level in the cortex (p<0.01) and increased 5-HIAA/5-HT rate (p<0.001) in the hypothalamus in B6-1473C mice, but not in B6-1473G mice. The present result is the first experimental evidence that C1473G polymorphism is involved in the regulation of the reaction to emotional stress in mice.