Details

Autor(en) / Beteiligte

• Patricia Rios-Ibarra, Clara
• Janeth Rodriguez-Silva, Claudia
• Alonso Lopez-Chuken, Yamil
• Ortiz-Lopez, Rocio
• Fernandez-Castillo, Elisa
• Beatriz Del Toro Runzer, Claudia
• Paul Armenta-Perez, Vicente
• Flores-Gutierrez, Juan Pablo
• Quintanilla-Guzman, Arturo
• Salinas-Santander, Mauricio
• Gonzalez-Guerrero, Juan Francisco
• Bosques-Padilla, Francisco
• Santos, Arturo
• Guadalupe Martinez-Rodriguez, Herminia

Titel

Thymidylate synthase polymorphism in Mexican patients with colon cancer treated with 5-fluorouracil

Ist Teil von

• Journal of B.U. ON., 2016-07, Vol.21 (4), p.935-940

Ort / Verlag

Greece

Erscheinungsjahr

2016

Quelle

MEDLINE

Beschreibungen/Notizen

• We analyzed the genotype and allele frequency of variable number tandem repeats (VNTR)-thymidylate synthase (TS) and its relationship with the disease evolution in colon cancer patients. We selected 24 paraffin-embedded colon cancer tissue samples from Mexican patients who received a 5-fluorouracil (5-FU)-based chemotherapy regimen. Tumor tissue was digested with proteinase K and genomic DNA was isolated by the standard method with phenol-chloroform extraction. Polymerase chain reaction (PCR) was performed for TS genotyping of VNTR and the results were evaluated directly in a stained agarose gel. The allele frequency of 2 repeats (2R) was greater (0.66) than 3R (0.34) in metastatic colon cancer (x =10.24; p=0.001)) however, no difference in allelic distribution between 2R (0.54) and 3R (0.46) in non metastatic patients was observed (x =0.640; p=0.424). Our results suggest that Mexican patients with colon cancer present differences in the allelic distribution, the 2R allele being the most frequent.