Details

Autor(en) / Beteiligte

• Kobayashi, Hiroto
• Yoshida, Saori
• Sun, Ying-Jie
• Shirasawa, Nobuyuki
• Naito, Akira

Titel

Gastric 17β-estradiol in portal vein and liver Esr1 make a circadian rhythm in systemic circulation in male rats

Ist Teil von

• Endocrine, 2016-08, Vol.53 (2), p.565-573

Ort / Verlag

New York: Springer US

Erscheinungsjahr

2016

Quelle

MEDLINE

Beschreibungen/Notizen

• The hemodynamics of 17β-estradiol (E 2 ) synthesized and secreted from the stomach has been revealed gradually. This study aimed to clarify the circadian rhythm of E 2 synthesis and secretion in the stomach, and the relationship between the expression of hepatic estrogen receptor (ER) α and serum E 2 levels in systemic circulation. Wistar male rats were maintained in a room with a 12-h light and 12-h dark cycle (lights on from 0700 to 1900 h), and were sacrificed at every 4-hour interval starting at 0800 h. The results showed that the expression of gastric Cyp19a1 was higher in nighttime than in daytime, and that the portal venous E 2 level was 2.2 times higher at 2400 h than that at 1200 h. The arterial E 2 level was also the highest at 2400 h, and showed an apparent circadian rhythm positively correlated with portal venous E 2 levels. Conversely, the expression of liver Esr1 peaked at 1200 h and turned to decrement at 2400 h. The population of immunoreactive nuclei with ERα antibody decreased at 2400 h compared with that at 1200 h. The regression analysis showed that the liver Esr1 mRNA was negatively correlated to portal venous and arterial E 2 levels. It could be concluded that the circadian rhythm of the systemic E 2 level depended both on the amounts of gastric E 2 in the portal vein and on the Esr1 expression in the liver.