Nature medicine, 2016-11, Vol.22 (11), p.1277-1284
2016
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- Autor(en) / Beteiligte
- Titel
- Translocation and dissemination of commensal bacteria in post-stroke infection
- Ist Teil von
- Nature medicine, 2016-11, Vol.22 (11), p.1277-1284
- Ort / Verlag
- United States
- Erscheinungsjahr
- 2016
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Bacterial infection is highly prevalent in patients who have had a stroke. Despite the potential contribution of micro-aspiration in post-stroke pneumonia, we found that the majority of the microorganisms detected in the patients who developed infections after having a stroke were common commensal bacteria that normally reside in the intestinal tracts. In a mouse model of ischemic stroke, post-stroke infection was only observed in mice that were born and raised in specific-pathogen-free facilities; this was not seen in mice that were born and raised in germ-free facilities. Using high-throughput 16S rRNA gene amplicon sequencing and bioinformatics analyses, we provide evidence demonstrating that the source of the bacteria forming the microbial community in the lungs of post-stroke mice was indeed the host small intestine. Additionally, stroke-induced gut barrier permeability and dysfunction preceded the dissemination of orally inoculated bacteria to peripheral tissues. This study identifies a novel pathway in which stroke promotes the translocation and dissemination of selective strains of bacteria that originated from the host gut microbiota.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1078-8956eISSN: 1546-170XDOI: 10.1038/nm.4194Titel-ID: cdi_crossref_primary_10_1038_nm_4194
- Format
- –
- Schlagworte
- Adrenergic beta-Antagonists - pharmacology, Aged, Aged, 80 and over, Animals, Bacteremia - immunology, Bacteremia - metabolism, Bacteremia - microbiology, Bacterial Infections - immunology, Bacterial Infections - metabolism, Bacterial Infections - microbiology, Bacterial Translocation - immunology, Blood Culture, Computational Biology, Disease Models, Animal, Enterococcus faecalis, Female, Gastrointestinal Microbiome - genetics, Goblet Cells - cytology, Goblet Cells - metabolism, Gram-Positive Bacterial Infections - immunology, Gram-Positive Bacterial Infections - metabolism, Gram-Positive Bacterial Infections - microbiology, High-Throughput Nucleotide Sequencing, Humans, Infarction, Middle Cerebral Artery - immunology, Intestine, Small - cytology, Intestine, Small - drug effects, Intestine, Small - metabolism, Intestine, Small - microbiology, Male, Mice, Microbiota - genetics, Middle Aged, Permeability - drug effects, Pneumonia, Bacterial - immunology, Pneumonia, Bacterial - metabolism, Pneumonia, Bacterial - microbiology, Receptors, Adrenergic, beta - metabolism, RNA, Ribosomal, 16S - genetics, Sequence Analysis, RNA, Specific Pathogen-Free Organisms, Stroke - immunology, Urinary Tract Infections - immunology, Urinary Tract Infections - metabolism, Urinary Tract Infections - microbiology, Zonula Occludens-1 Protein - metabolism