Details

Autor(en) / Beteiligte

• Chang, Irene Y. T.
• Joester, Derk

Titel

Large Area Cryo-Planing of Vitrified Samples Using Broad-Beam Ion Milling

Ist Teil von

• Microscopy and microanalysis, 2015-12, Vol.21 (6), p.1616-1621

Ort / Verlag

New York, USA: Cambridge University Press

Erscheinungsjahr

2015

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• On account of its excellent resolution and high throughput, cryoSEM imaging has recently seen resurgence. In this work, we report on the development of cryogenic triple ion gun milling (CryoTIGM™), a broad ion beam milling technique for cryo-planing of vitrified, “frozen-hydrated” specimens. We find that sections prepared with CryoTIGM™ are smooth over exceptionally large areas (~700,000 µm2), and reveal ultrastructural details in similar or better quality than freeze-fractured samples.