Details

Autor(en) / Beteiligte

• Chang, J.
• Frandsen, S.
• Gadsby, J.E.

Titel

Prostaglandin synthesis by the porcine corpus luteum: effect of tumor necrosis factor-α

Ist Teil von

• Domestic animal endocrinology, 2017-01, Vol.58, p.53-62

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2017

Quelle

MEDLINE

Beschreibungen/Notizen

• The porcine corpus luteum (CL) displays delayed sensitivity to PGF-2α (luteolytic sensitivity, [LS]) until days 12 to 13 of cycle. The control of LS is unknown, but it is temporally associated with macrophage (which secrete tumor necrosis factor-α; TNF-α) infiltration into the CL. Other studies showed that TNF-α induces LS in vitro and that prostaglandins (PGs) may be involved in this mechanism. In experiment 1, PGF-2α and PGE secretion by luteal cells (LCs) was measured on days 4 to 14 of the estrous cycle, and the expression of PTGFS/AKR1B1 and PTGES/mPGES-1, determined by Western blot, before (day 7) vs after (day 13) the onset of LS. Results showed that the PGF-2α:PGE ratio increased significantly (P < 0.05) from day 4 to 13–14, and PTGFS/AKR1B1 and PTGES/mPGES-1 were significantly increased (P < 0.05) on day 13 (vs day 7). In experiment 2, LCs were collected from porcine CL at early (∼days 4–6) or mid (∼days 7–12) stages of the estrous cycle and cultured with 0, 0.1, 1, or 10 ng/mL TNF-α. Results showed that TNF-α significantly increased (P < 0.05) messenger RNA (mRNA) expression of cyclooxygenase (COX)-2 and mPGES-1 but not AKR1B1. TNF-α had no significant effects on AKR1B1 or mPGES protein abundance. TNF-α significantly increased (P < 0.05) PGE-2 but had no effect on PGF-2α secretion or on the PGF-2α:PGE2 ratio. In conclusion, although TNF-α increased COX2 and mPGES-1 mRNA, and PGE-2 secretion in vitro, it did not increase the PGF-2α:PGE2 ratio. Studies are currently directed toward exploring other pathways (eg, FP receptor signaling) by which TNF-α induces LS in the porcine CL. •Prostaglandin (PG)F-2a:PGE ratio increased (P < 0.05) from day 4 to 13-14 of cycle.•PGF (AKR1B1) & PGE (mPGES-1) Synthases were increased (P < 0.05) on day 13 (vs day 7).•Tumor necrosis factor (TNF)-a increased (P < 0.05) mPGES-1, but not AKR1B1 mRNAs.•TNF-a had no significant effects on AKR1B1 or mPGES-1 protein abundance.•TNF-a significantly increased PGE-2 but had no effect on PGF-2a or PGF-2a:PGE-2 ratio.