Details

Autor(en) / Beteiligte

• Ma, Xiaoxiao
• Zhao, Xu
• Li, Junjie
• Zhang, Wenpeng
• Cheng, Ji-Xin
• Ouyang, Zheng
• Xia, Yu

Titel

Photochemical Tagging for Quantitation of Unsaturated Fatty Acids by Mass Spectrometry

Ist Teil von

• Analytical chemistry (Washington), 2016-09, Vol.88 (18), p.8931-8935

Ort / Verlag

United States: American Chemical Society

Erscheinungsjahr

2016

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Fatty acid (FA) profiling provides phenotypic information and is increasingly used in a broad range of biological and biomedical studies. Quantitation of unsaturated FAs with confident carbon–carbon double bond (CC) location assignment is both sample and time consuming using traditional gas chromatography mass spectrometry analysis. In this study, we developed a rapid, sensitive, and quantitative method for profiling unsaturated FAs without using chromatographic separations. This method was based on a combination of in-solution photochemical tagging of a CC in FAs and a subsequent gas-phase detagging via tandem (neutral loss scan) mass spectrometry. It enabled quantitation of unsaturated FAs from various biological samples (blood, plasma, and cell lines). More importantly, quantitative information on FA CC location isomers, which was traditionally overlooked, could now be obtained and applied to studying FA changes between normal and cancerous human prostate cells.