Cell stem cell, 2017-03, Vol.20 (3), p.360-373.e7
2017
Details
- Autor(en) / Beteiligte
- Titel
- Neurogenic Radial Glia-like Cells in Meninges Migrate and Differentiate into Functionally Integrated Neurons in the Neonatal Cortex
- Ist Teil von
- Cell stem cell, 2017-03, Vol.20 (3), p.360-373.e7
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2017
- Link zum Volltext
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Whether new neurons are added in the postnatal cerebral cortex is still debated. Here, we report that the meninges of perinatal mice contain a population of neurogenic progenitors formed during embryonic development that migrate to the caudal cortex and differentiate into Satb2+ neurons in cortical layers II–IV. The resulting neurons are electrically functional and integrated into local microcircuits. Single-cell RNA sequencing identified meningeal cells with distinct transcriptome signatures characteristic of (1) neurogenic radial glia-like cells (resembling neural stem cells in the SVZ), (2) neuronal cells, and (3) a cell type with an intermediate phenotype, possibly representing radial glia-like meningeal cells differentiating to neuronal cells. Thus, we have identified a pool of embryonically derived radial glia-like cells present in the meninges that migrate and differentiate into functional neurons in the neonatal cerebral cortex. [Display omitted] •Lineage tracing identifies a meningeal neural progenitor (NP) population•Meningeal NPs migrate from the meninges to the cortex in the neonatal brain•Meningeal NPs differentiate into functionally integrated cortical neurons•Single-cell profiling highlights radial glia-like characteristics of meningeal NPs Carmeliet and colleagues have identified a population of radial glia-like neural progenitor cells located in the meninges of postnatal mice. After birth, these cells migrate through the caudal ventricular zone and then differentiate into functional and integrated neurons of the posterior cortex.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1934-5909eISSN: 1875-9777DOI: 10.1016/j.stem.2016.10.020Titel-ID: cdi_proquest_miscellaneous_1844354200
- Format
- –
- Schlagworte
- Animals, Animals, Newborn, Cell Differentiation, Cell Lineage, Cell Movement, Cerebral Cortex - cytology, electrophysiology, Embryo, Mammalian - cytology, Excitatory Amino Acid Transporter 1 - metabolism, Gene Expression Profiling, HEK293 Cells, Humans, lineage tracing, meninges, Meninges - cytology, Mice, Inbred C57BL, neonatal cerebral cortex, Nestin - metabolism, neural progenitors, Neurogenesis, Neuroglia - cytology, Neurons - cytology, PDGFRβ, radial glia cells, Receptor, Platelet-Derived Growth Factor beta - metabolism, Reproducibility of Results, Single-Cell Analysis, single-cell RNA sequencing, Spheroids, Cellular - cytology, Staining and Labeling, Transcriptome - genetics