Details

Autor(en) / Beteiligte

• Kalimeris, Konstantinos
• Briassoulis, Panagiotis
• Ntzouvani, Agathi
• Nomikos, Tzortzis
• Papaparaskeva, Kleio
• Politi, Aikaterini
• Batistaki, Chrysanthi
• Kostopanagiotou, Georgia

Titel

N-acetylcysteine ameliorates liver injury in a rat model of intestinal ischemia-reperfusion

Ist Teil von

• The Journal of surgical research, 2016-12, Vol.206 (2), p.263-272

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2016

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Abstract Background N-acetylcysteine (NAC) is an antioxidant with direct and indirect antioxidant actions used in the clinical setting. Oxidative stress is known to play a pivotal role in the intestinal ischemia-reperfusion (IIR). Therefore we studied the effect of different pretreatment regimens with NAC on the IIR injury in rats. Materials & methods Thirty-five male Wistar rats were randomly assigned to 5 groups. In group SHAM only laparotomy was performed. Group CONTROL underwent IIR without NAC. In the other groups, NAC was administered intraperitoneally with different regimens: 150 mg/kg before ischemia (NAC150), 300 mg/kg before ischemia (NAC300) and 150 mg/kg before ischemia plus 150 mg/kg 5 min before reperfusion (NAC150+150). Measurements in tissues and blood were conducted at 4 hours of reperfusion following exsanguination. Results Histological score of the liver was significantly improved in NAC300 compared with control (1.7±0.5 vs. 2.9±1.1 respectively, p=0.05). In addition, NAC treatment significantly reduced liver transaminases in all groups of treatment, mostly in group NAC300. Plasma malondialdehyde levels were lower with NAC treatment, although not statistically significantly. Lung glutathione peroxidase was significantly increased in group NAC300 (p=0.04), while the other oxidation biomarkers showed no significant differences. Conclusion NAC exerts a significant protective role in liver injury following IIR, which seems to be independent of an intestinal protective effect. Additional administration of NAC before reperfusion was of no further benefit. The most effective regimen among the compared regimens was that of 300 mg/kg before ischemia.