Details

Autor(en) / Beteiligte

• Cheplakova, Anastasia M.
• Solovieva, Anastasiya O.
• Pozmogova, Tatiana N.
• Vorotnikov, Yuri A.
• Brylev, Konstantin A.
• Vorotnikova, Natalya A.
• Vorontsova, Elena V.
• Mironov, Yuri V.
• Poveshchenko, Alexander F.
• Kovalenko, Konstantin A.
• Shestopalov, Michael A.

Titel

Nanosized mesoporous metal–organic framework MIL-101 as a nanocarrier for photoactive hexamolybdenum cluster compounds

Ist Teil von

• Journal of inorganic biochemistry, 2017-01, Vol.166, p.100-107

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2017

Quelle

MEDLINE

Beschreibungen/Notizen

• Inclusion compounds of photoluminescent hexamolybdenum cluster complexes in the chromium terephthalate metal-organic framework, MIL-101 (MIL, Matérial Institut Lavoisier) were successfully synthesized in two different ways and characterized by means of powder X-Ray diffraction, chemical analysis and nitrogen sorption. Some important functional properties of hexamolybdenum cluster complexes for biological and medical applications, in particular singlet oxygen generation ability, luminescence properties, cellular uptake behavior and cytotoxicity were studied. It was revealed that the inclusion compounds possessed significant singlet oxygen generation activity. The materials obtained showed a low cytotoxicity, thus allowing them to be used in living cells. Confocal microscopy of human larynx carcinoma (Hep-2) cells incubated with the inclusion compounds showed that MIL-101 performed as a nanocarrier adhering to the external cell membrane surface and releasing the cluster complexes which that penetrated into the cells. Moreover, photoinduced generation of reactive oxygen species (ROS) in Hep-2 cells incubated with inclusion compounds was demonstrated. The cluster supported on MIL-101 was shown to possess in vivo phototoxicity. Hexamolybdenum clusters possessing red fluorescence were introduced into nanosized chromium terephthalate metal-organic framework. Clusters anchored via pyrazine cannot penetrate into cells whereas clusters from pyrazine-free compound transport through membrane and generate singlet oxygen in cancer Hep-2 cells. Thus, such system is a suitable nanocarrier for applications in photodynamic therapy. [Display omitted] •Molybdenum clusters in mesoporous chromium terephthalate metal-organic framework.•Luminescence of hybrids were investigated and compared to clusters in solutions.•Cytotoxicity of all compounds were established at low level.•Penetration of clusters into Hep-2 cells were observed for pyrazine-free compound.•Generation of reactive oxygen species were tested in vitro and in living cells.