Details

Autor(en) / Beteiligte

• TOTET, ANNE
• LATOUCHE, SOPHIE
• LACUBE, PHILIPPE
• BOLOGNINI, JOSIANE
• RACCURT, CHRISTIAN
• NEVEZ, GILLES
• ROUX, PATRICIA

Titel

Typing of Pneumocystis carinii f.sp. hominis Isolates from Nasopharyngeal Aspirates of Immunocompetent Infants with Bronchiolitis by Dihydropteroate Synthase Gene Analysis

Ist Teil von

• The Journal of eukaryotic microbiology, 2001-06, Vol.48 (s1), p.121s-121s

Ort / Verlag

Oxford, UK: Blackwell Publishing Ltd

Erscheinungsjahr

2001

Quelle

Wiley Online Library Journals Frontfile Complete

Beschreibungen/Notizen

• Sulfa and sulfone drugs are widely used in Pneumocystis carinii pneumonia (PCP) prophylaxis and treatment. Their enzymatic target is the dihydropteroate synthase (DHPS). Point mutations have been described in Pneumocystis carinii f.sp. hominis (P.c.hominis) DHPS gene, mainly at positions 165 and 171. Mutant P.c.hominis DHPS genotypes have been detected in patients developing PCP with or without prior sulfa or sulfone prophylaxis. In a recent study, it was shown that P.c.hominis infected 45 immunocompetent infants diagnosed with bronchiolitis. The fungus was detected in nasopharyngeal aspirates (NPAs) using a nested PCR assay amplifying a portion of the mitochondrial large sub-unit rRNA (mtLSUrRNA) gene. None of these infants received sulfa or sulfone drugs before and after P.c. hominis DNA detection. The aim of this study was to identify wild and mutant P.c. hominis DHPS genotypes in this infant population.