Details

Autor(en) / Beteiligte

• Wei, Wei
• Xu, Chao
• Ye, Zhi-Yong
• Huang, Xiao-Jun
• Yuan, Jia-En
• Ma, Tian-Bao
• Lin, Han-Biao
• Chen, Xiu-Qiong

Titel

Biological characteristics of mesenchymal stem cell and hematopoietic stem cell in the co-culture system

Ist Teil von

• Sheng li hsüeh pao, 2016-10, Vol.68 (5), p.691-698

Ort / Verlag

China

Erscheinungsjahr

2016

Quelle

MEDLINE

Beschreibungen/Notizen

• The aim of the present study was to obtain the qualified hematopoietic stem/progenitor cells (HSC/HPC) and human umbilical cord-mesenchymal stem cells (MSC) in vitro in the co-culture system. Cord blood mononuclear cells were separated from umbilical cord blood by Ficoll lymphocyte separation medium, and then CD34 HSC was collected by MACS immunomagnetic beads. The selected CD34 HSC/HPC and MSC were transferred into culture flask. IMDM culture medium with 15% AB-type cord plasma supplemented with interleukin-3 (IL-3), IL-6, thrombopoietin (TPO), stem cell factor (SCF) and FMS-like tyrosine kinase 3 ligand (Flt-3L) factors were used as the co-culture system for the amplification of HSC/HPC and MSC. The cellular growth status and proliferation on day 6 and 10 after co-culture were observed by using inverted microscope. The percentage of positive expression of CD34 in HSC/HPC, as well as the percentages of positive expressions of CD105, CD90, CD73, CD45, CD34 and HLA-DR in the 4 generation MSC, was tested by flo