Details

Autor(en) / Beteiligte

• Jin, Shi-ying
• Li, Rui-sheng
• Shen, Bao-de
• Bai, Jin-xia
• Xu, Ping-hua
• Dai, Ling
• Xu, He
• Jin, Shi-xiao
• Han, Jin
• Yuan, Hai-long

Titel

Lignans-Rich Extract from Herpetospermum caudigerum Alleviate Physical Fatigue in Mice

Ist Teil von

• Chinese journal of integrative medicine, 2016-11, Vol.22 (11), p.840-845

Ort / Verlag

Berlin/Heidelberg: Springer Berlin Heidelberg

Erscheinungsjahr

2016

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Objective： To ascertain anti-fatigue constituents and mechanisms of Herpetospermum caudigerum.Methods： The 80% ethanol extracts of Herpetospermum caudigerum were partitioned with chloroform, ethyl acetateand n-butanol, respectively. Male Kunming mice were divided into 13 groups with 16 mice in each group： a controlgroup fed with water, 9 groups treated with 3 fractions of Herpetospermum caudigerum （chloroform fraction, ethylacetate fraction and n-butanol fraction） at dose of 80, 160 and 320 mg/kg for the low-dose group, medium-dosegroup and high-dose group, 3 herpetrione （HPE） treated groups fed with HPE at dose of 15, 30, and 60 mg/kg forthe low-dose group, medium-dose group and high-dose group. All animals were treated once per day for 30 days.Anti-fatigue activity was assessed through the forced swimming test and serum biochemical parameters includingblood lactic acid （BLA）, blood urea nitrogen （BUN）, malondialdehyde （MDA）, hepatic glycogen （HG）, lacticdehydrogenase （LDH）, superoxide dismutase （SOD） and glutathione peroxidase （GPx） determined followingthe recommended procedures provided by the commercial kits. Results： Compared with the control group, thelignans extract （ethyl acetate fraction） of Herpetospermum caudigerum and HPE could significantly prolongedthe exhaustive swimming time （P〈0.05 or ,O〈0.01）, and also increased the HG levels （P〈0.05 or ,P〈0.01） and theactivities of antioxidant enzymes （SOD, GPx and LDH, P〈0.05 or ,P〈0.01）; BLA and MDA levels were decreasedconsiderably in lignans extract and HPE treated groups （P〈0.05 or ,P〈0.01）. HPE also could significantlydecrease the BUN contents compared with the control group （P〈0.05）. The chloroform and n-butanol fractionshowed no effect on swimming time and biochemical parameters. Conclusions： The lignans extract had anti-fatigue activities and HPE may be partly responsible for the anti-fatigue effects of Herpetospermum caudigerum.The possible mechanisms of anti-fatigue activity were related to the decrease of BUN and BLA, the increaseof the HG storage and protecting corpuscular membrane by preventing lipid oxidation via modifying severalenzyme activities.