Details

Autor(en) / Beteiligte

• Ji, Li-li
• Guo, Meng-wei
• Ren, Xiu-jun
• Ge, Dong-yu
• Li, Gen-mao
• Tu, Ya

Titel

Effects of Electroacupuncture Intervention on Expression of Cyclooxygenase 2 and Microglia in Spinal Cord in Rat Model of Neuropathic Pain

Ist Teil von

• Chinese journal of integrative medicine, 2017-10, Vol.23 (10), p.786-792

Ort / Verlag

Berlin/Heidelberg: Springer Berlin Heidelberg

Erscheinungsjahr

2017

Quelle

Springer Online Journals【Remote access available】

Beschreibungen/Notizen

• Objective： To investigate the effect of electroacupuncture（EA） treatment on the expression of cyclooxygenase（COX） 2 and microglia in spinal cord by using rat model of neuropathic pain, and to probe into the relationship between COX 2 and microglia. Methods： The rats were randomly divided into 6 groups, including normal control group, model group, sham group, EA 1 group（distant acupoints ＋ local acupoints）, EA 2 group（local acupoints）, and EA 3 group（distant acupoints）. Thermal withdrawal latencies were evaluated at 1 day preoperatively and 3, 5 and 7 days postoperatively. At 7 days postoperatively, the spinal COX 2 m RNA was detected by reverse-transcription polymerase chain reaction. Double immunofluorescent staining technology was applied to screen and verify the relationship between altered COX 2 and microglia. Results： Compared with the model group, thermal withdrawal latencies increased after EA treatment（P〈0.01）. The expressions of COX 2 m RNA were up-regulated in spinal cord of rat on day 7 after surgery（P〈0.05）. Compared with the model group, EA stimulation（EA 1 and EA 2 groups） reversed the up-regulation of COX 2 m RNA expression（P〈0.05）. EA 1 and EA 2 groups might have better treatment effect compared with the EA 3 group. Fluorescent images displayed COX 2 and microglia expressed at common areas. Conclusions： EA was effective in analgesic and anti-inflammatory. EA has decreased the expression of spinal COX 2 m RNA in the trend of the therapeutic effect of ＂distant acupoints ＋ local acupoints＂, and ＂local acupoints＂ intervention may be superior to that of ＂distant acupoints＂ intervention. Microglia may be related to the formation of COX 2.