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Effects of Electroacupuncture Intervention on Expression of Cyclooxygenase 2 and Microglia in Spinal Cord in Rat Model of Neuropathic Pain
Ist Teil von
Chinese journal of integrative medicine, 2017-10, Vol.23 (10), p.786-792
Ort / Verlag
Berlin/Heidelberg: Springer Berlin Heidelberg
Erscheinungsjahr
2017
Quelle
Springer Online Journals【Remote access available】
Beschreibungen/Notizen
Objective: To investigate the effect of electroacupuncture(EA) treatment on the expression of cyclooxygenase(COX) 2 and microglia in spinal cord by using rat model of neuropathic pain, and to probe into the relationship between COX 2 and microglia. Methods: The rats were randomly divided into 6 groups, including normal control group, model group, sham group, EA 1 group(distant acupoints + local acupoints), EA 2 group(local acupoints), and EA 3 group(distant acupoints). Thermal withdrawal latencies were evaluated at 1 day preoperatively and 3, 5 and 7 days postoperatively. At 7 days postoperatively, the spinal COX 2 m RNA was detected by reverse-transcription polymerase chain reaction. Double immunofluorescent staining technology was applied to screen and verify the relationship between altered COX 2 and microglia. Results: Compared with the model group, thermal withdrawal latencies increased after EA treatment(P〈0.01). The expressions of COX 2 m RNA were up-regulated in spinal cord of rat on day 7 after surgery(P〈0.05). Compared with the model group, EA stimulation(EA 1 and EA 2 groups) reversed the up-regulation of COX 2 m RNA expression(P〈0.05). EA 1 and EA 2 groups might have better treatment effect compared with the EA 3 group. Fluorescent images displayed COX 2 and microglia expressed at common areas. Conclusions: EA was effective in analgesic and anti-inflammatory. EA has decreased the expression of spinal COX 2 m RNA in the trend of the therapeutic effect of "distant acupoints + local acupoints", and "local acupoints" intervention may be superior to that of "distant acupoints" intervention. Microglia may be related to the formation of COX 2.