Details

Autor(en) / Beteiligte

• Miao, Hong
• Wang, Lan
• Zhuo, Yan
• Zhou, Zinan
• Yang, Xiaoming

Titel

Label-free fluorimetric detection of CEA using carbon dots derived from tomato juice

Ist Teil von

• Biosensors & bioelectronics, 2016-12, Vol.86, p.83-89

Ort / Verlag

England: Elsevier B.V

Erscheinungsjahr

2016

Quelle

MEDLINE

Beschreibungen/Notizen

• A facile-green strategy to synthesize carbon dots (CDs) with a quantum yield (QY) of nearly 13.9% has been built up, while tomato juice served as the carbon source. Interestingly, not only the precursor of CDs and the whole synthesis procedure were environmental-friendly, but this type of CDs also exhibited multiple advantages including high fluorescent QY, excellent photostability, non-toxicity and satisfactory stability. Significantly, a label-free sensitive assay for detecting carcinoembryonic antigen (CEA) in a continuous and recyclable way has been proposed on the basis of adsorption and desorption of aptamers by the surface of CDs through a competitive mechanism. To be specific, the richness of carboxyl groups of the CDs enabled strong adsorption of ssDNA to the surface of CDs through π-π stacking interactions, resulting in the effective fluorescence quenching by forming CDs-aptamer complexes. The stronger binding affinity between CEA and CEA-aptamer than the π-π stacking interactions has been taken advantage to achieve immediate recovery of the fluorescence of CDs once CEA was introduced. Thereby, quantitative evaluation of CEA concentration in a broad range from 1ngmL−1 to 0.5ngmL−1 with the detection limit of 0.3ngmL−1 was realized in this way. This strategy can be applied in a recyclable way, broadening the sensing application of CDs with biocompatibility. Besides, the CDs were used for cell imaging, potentiating them towards diverse purposes. •Carbon dots (CDs) derived from tomato juice were first synthesized.•A label-free assay for CEA and CEA-aptamer has been established based on this CDs.•Photoinduced electron transfer was established to explore the quenching mechanism.•This strategy has broadening the sensing application of CDs with biocompatibility.