Details

Autor(en) / Beteiligte

• Zhang, Y
• Yang, X
• Zhou, F Y
• Zhang, A F
• Zhu, X F
• Chen, Y
• Zhou, M G
• Gao, T C

Titel

Detection of a mutation at codon 43 of the rps L gene in Xanthomonas oryzae pv. oryzicola and X. oryzae pv. oryzae by PCR-RFLP

Ist Teil von

• Genetics and molecular research, 2015-01, Vol.14 (4), p.18587-18595

Erscheinungsjahr

2015

Quelle

Electronic Journals Library

Beschreibungen/Notizen

• The aim of this study was to develop a method to detect a point mutation in the ribosomal S12 protein (rps L) gene in streptomycin-resistant strains of Xanthomonas oryzae pv. oryzicola and X. oryzae pv. oryzae. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was developed to detect a point mutation in codon 43 of the rps L gene in X. oryzae pv. oryzicola and X. oryzae pv. oryzae. The 304-bp PCR product from the rps L gene was digested by Mbo II to form two fragments (201 and 103 bp) if there was a mutation at codon 43, or three fragments (146, 103, and 55 bp) if there was no mutation. Compared with the results from nucleotide sequencing, the PCR-RFLP method was accurate in detecting the point mutation at codon 43 of the rps L gene in streptomycin-resistant strains of X. oryzae pv. oryzicola and X. oryzae pv. oryzae. These results indicate that the PCR-RFLP is a simple, rapid and reliable method for detecting the point mutation at codon 43 of the rps L gene.