Details

Autor(en) / Beteiligte

• Kwon, Daekee
• Koo, Ok-Jae
• Kim, Min-Jung
• Jang, Goo
• Lee, Byeong Chun

Titel

Nuclear-mitochondrial incompatibility in interorder rhesus monkey–cow embryos derived from somatic cell nuclear transfer

Ist Teil von

• Primates, 2016-10, Vol.57 (4), p.471-478

Ort / Verlag

Tokyo: Springer Japan

Erscheinungsjahr

2016

Quelle

MEDLINE

Beschreibungen/Notizen

• Monkey interorder somatic cell nuclear transfer (iSCNT) using enucleated cow oocytes yielded poor blastocysts development and contradictory results among research groups. Determining the reason for this low blastocyst development is a prerequisite for optimizing iSCNT in rhesus monkeys. The aim of this study was to elucidate nuclear-mitochondrial incompatibility of rhesus monkey-cow iSCNT embryos and its relationship to low blastocyst development. Cytochrome b is a protein of complex III of the electron transport chain (ETC). According to meta-analysis of amino acid sequences, the homology of cytochrome b is 75 % between rhesus monkeys and cattle. To maintain the function of ETC after iSCNT, 4n iSCNT embryos were produced by fusion of non-enucleated cow oocytes and rhesus monkey somatic cells. The blastocyst development rate of 4n iSCNT embryos was higher than that of 2n embryos ( P  < 0.01). Formation of reactive oxygen species (ROS) is an indirect indicator of ETC activity of cells. The ROS levels of 4n iSCNT embryos was higher than that of 2n embryos ( P  < 0.01). Collectively, rhesus monkey iSCNT embryos reconstructed with cow oocytes have nuclear-mitochondrial incompatibility due to fundamental species differences between rhesus monkeys and cattle. Nuclear-mitochondrial incompatibility seems to correlate with low ETC activity and extremely low blastocyst development of rhesus monkey-cow iSCNT embryos.