Details

Autor(en) / Beteiligte

• Aydinoglu, Sabriye
• Biver, Tarita
• Figuccia, Stefania
• Fiore, Tiziana
• Montanaro, Sonia
• Pellerito, Claudia

Titel

Studies on DNA interaction of organotin(IV) complexes of meso-tetra(4-sulfonatophenyl)porphine that show cellular activity

Ist Teil von

• Journal of inorganic biochemistry, 2016-10, Vol.163, p.311-317

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2016

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• The interaction of the diorgano- and triorganotin(IV) derivatives of meso-tetra-(4-sulfonatophenyl)porphine (Me2Sn)2TPPS, (Bu2Sn)2TPPS, (Me3Sn)4TPPS and (Bu3Sn)4TPPS to natural DNA was analysed (together with free meso-tetra-(4-sulfonatophenyl)porphine (TPPS4−) for comparison purposes). Particular attention was paid to (Bu3Sn)4TPPS, a species that shows significant cellular action. Preliminary tests were done on the solution properties of the organotin(IV) compounds (pKA and possible self-aggregation). Spectrophotometric and spectrofluorometric experiments showed that all the investigated organotin(IV) derivatives strongly interact with DNA, the binding energy depending on the dye steric hindrance. In all cases experimental data concur in indicating that external binding mode prevails. Interestingly, fluorescence quenching and viscosity experiments show that the Bu-containing species, and in particular (Bu3Sn)4TPPS, are able to noticeably alter the DNA conformation. Diorgano- and triorganotin(IV) derivatives of meso-tetra-(4-sulfonatophenyl)porphine interact with DNA and the butyl-containing species, known from literature to be endowed with significant cellular activity, are found to noticeably alter the DNA conformation. [Display omitted] •Organotin substituents induce slight decrease of pKA of the central porphyrin ring.•Aggregation of the dyes on the DNA surface does occur for high dye content.•In excess of DNA the dyes monomers do bind DNA in external binding mode.•DNA conformation is altered upon binding.•The DNA modifications are more important in the presence of butyl residues.