Details

Autor(en) / Beteiligte

• Luchkina, Natalia V.
• Coleman, Sarah K.
• Huupponen, Johanna
• Cai, Chunlin
• Kivistö, Anna
• Taira, Tomi
• Keinänen, Kari
• Lauri, Sari E.

Titel

Molecular mechanisms controlling synaptic recruitment of GluA4 subunit-containing AMPA-receptors critical for functional maturation of CA1 glutamatergic synapses

Ist Teil von

• Neuropharmacology, 2017-01, Vol.112 (Pt A), p.46-56

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2017

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Synaptic recruitment of AMPA receptors (AMPARs) represents a key postsynaptic mechanism driving functional development and maturation of glutamatergic synapses. At immature hippocampal synapses, PKA-driven synaptic insertion of GluA4 is the predominant mechanism for synaptic reinforcement. However, the physiological significance and molecular determinants of this developmentally restricted form of plasticity are not known. Here we show that PKA activation leads to insertion of GluA4 to synaptic sites with initially weak or silent AMPAR-mediated transmission. This effect depends on a novel mechanism involving the extreme C-terminal end of GluA4, which interacts with the membrane proximal region of the C-terminal domain to control GluA4 trafficking. In the absence of GluA4, strengthening of AMPAR-mediated transmission during postnatal development was significantly delayed. These data suggest that the GluA4-mediated activation of silent synapses is a critical mechanism facilitating the functional maturation of glutamatergic circuitry during the critical period of experience-dependent fine-tuning. This article is part of the Special Issue entitled ‘Ionotropic glutamate receptors’. •PKA activation leads to synaptic unsilencing via insertion of GluA4.•This depends on a novel mechanism involving the extreme C-terminal end of GluA4.•Absence of GluA4 causes delayed postnatal maturation of AMPA transmission.