Details
- Autor(en) / Beteiligte
- Titel
- Microbiological laboratory diagnostics of neglected zoonotic diseases (NZDs)
- Ist Teil von
- Acta tropica, 2017-01, Vol.165, p.40-65
- Ort / Verlag
- Netherlands: Elsevier B.V
- Erscheinungsjahr
- 2017
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- According to the subjective opinion of the authors, the graphical abstract shows the importance of 4 different laboratory diagnostic approaches (microscopical-, cultural-, molecular biological- and serological approaches) for leishmaniasis, echinococcosis, cysticercosis, trypanosomiasis, tuberculosis, rabies, brucellosis and anthrax. [Display omitted] •Anthrax.•Bovine tuberculosis.•Brucellosis.•Echinococcosis.•Leishmaniasis.•Rabies.•Taenia solium-associated diseases (neuro-/cysticercosis & taeniasis).•Trypanosomiasis. This review reports on laboratory diagnostic approaches for selected, highly pathogenic neglected zoonotic diseases, i.e. anthrax, bovine tuberculosis, brucellosis, echinococcosis, leishmaniasis, rabies, Taenia solium-associated diseases (neuro-/cysticercosis & taeniasis) and trypanosomiasis. Diagnostic options, including microscopy, culture, matrix-assisted laser-desorption-ionisation time-of-flight mass spectrometry, molecular approaches and serology are introduced. These procedures are critically discussed regarding their diagnostic reliability and state of evaluation. For rare diseases reliable evaluation data are scarce due to the rarity of samples. If bio-safety level 3 is required for cultural growth, but such high standards of laboratory infrastructure are not available, serological and molecular approaches from inactivated sample material might be alternatives. Multiple subsequent testing using various test platforms in a stepwise approach may improve sensitivity and specificity. Cheap and easy to use tests, usually called “rapid diagnostic tests” (RDTs) may impact disease control measures, but should not preclude developing countries from state of the art diagnostics.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0001-706XeISSN: 1873-6254DOI: 10.1016/j.actatropica.2015.09.003Titel-ID: cdi_proquest_miscellaneous_1826640299
- Format
- –
- Schlagworte
- Animals, Cattle, Clinical Laboratory Techniques - methods, Culture, Diagnostic, Humans, Laboratory, Microscopy, Molecular diagnosis, Molecular Diagnostic Techniques, Neglected Diseases - diagnosis, Neglected tropical disease, Neglected zoonotic disease, NZD, PCR, Reliability, Reproducibility of Results, Sensitivity, Sensitivity and Specificity, Serologic Tests, Serology, Specificity, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Zoonoses - diagnosis