Details

Autor(en) / Beteiligte

• Benevento, Marco
• Tonge, Peter D.
• Puri, Mira C.
• Hussein, Samer M. I.
• Cloonan, Nicole
• Wood, David L.
• Grimmond, Sean M.
• Nagy, Andras
• Munoz, Javier
• Heck, Albert J. R.

Titel

Proteome adaptation in cell reprogramming proceeds via distinct transcriptional networks

Ist Teil von

• Nature communications, 2014-12, Vol.5 (1), p.5613-5613, Article 5613

Ort / Verlag

London: Nature Publishing Group UK

Erscheinungsjahr

2014

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• The ectopic expression of Oct4, Klf4, c-Myc and Sox2 (OKMS) transcription factors allows reprogramming of somatic cells into induced pluripotent stem cells (iPSCs). The reprogramming process, which involves a complex network of molecular events, is not yet fully characterized. Here we perform a quantitative mass spectrometry-based analysis to probe in-depth dynamic proteome changes during somatic cell reprogramming. Our data reveal defined waves of proteome resetting, with the first wave occurring 48 h after the activation of the reprogramming transgenes and involving specific biological processes linked to the c-Myc transcriptional network. A second wave of proteome reorganization occurs in a later stage of reprogramming, where we characterize the proteome of two distinct pluripotent cellular populations. In addition, the overlay of our proteome resource with parallel generated -omics data is explored to identify post-transcriptionally regulated proteins involved in key steps during reprogramming. During somatic cell reprogramming, the cell transits through intermediate states. Here, the authors perform an in-depth quantitative proteomic analysis of the reprogramming of mouse embryonic fibroblasts to induced pluripotent stem cells and observe two waves of proteome reorganisation.