Cell, 2002-01, Vol.108 (1), p.121-133
2002
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- Autor(en) / Beteiligte
- Titel
- β Phorbol Ester- and Diacylglycerol-Induced Augmentation of Transmitter Release Is Mediated by Munc13s and Not by PKCs
- Ist Teil von
- Cell, 2002-01, Vol.108 (1), p.121-133
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2002
- Quelle
- Elsevier ScienceDirect Journals
- Beschreibungen/Notizen
- Munc13-1 is a presynaptic protein with an essential role in synaptic vesicle priming. It contains a diacylglycerol (DAG)/β phorbol ester binding C 1 domain and is a potential target of the DAG second messenger pathway that may act in parallel with PKCs. Using genetically modified mice that express a DAG/β phorbol ester binding-deficient Munc13-1 H567K variant instead of the wild-type protein, we determined the relative contribution of PKCs and Munc13-1 to DAG/β phorbol ester-dependent regulation of neurotransmitter release. We show that Munc13s are the main presynaptic DAG/β phorbol ester receptors in hippocampal neurons. Modulation of Munc13-1 activity by second messengers via the DAG/β phorbol ester binding C 1 domain is essential for use-dependent alterations of synaptic efficacy and survival.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0092-8674eISSN: 1097-4172DOI: 10.1016/S0092-8674(01)00635-3Titel-ID: cdi_proquest_miscellaneous_18262446
- Format
- –
- Schlagworte
- Amino Acid Sequence, Animals, Base Sequence, Binding Sites - genetics, Cells, Cultured, diacylglycerol, Diglycerides - metabolism, Gene Expression - physiology, Hippocampus - cytology, Intracellular Signaling Peptides and Proteins, Mice, Molecular Sequence Data, Munc13-1 protein, Mutagenesis - physiology, Nerve Tissue Proteins - genetics, Nerve Tissue Proteins - metabolism, Neurons - cytology, Phorbol Esters - pharmacology, Point Mutation - physiology, Protein Kinase C - genetics, Protein Kinase C - metabolism, Synapses - enzymology, Synaptic Transmission - drug effects, Synaptic Transmission - physiology