Details

Autor(en) / Beteiligte

• Li, Yi-Tong
• Huang, Yi-Chao
• Xu, Yang
• Pan, Man
• Li, Yi-Ming

Titel

Ubiquitin 7-amino-4-carbamoylmethylcoumarin as an improved fluorogenic substrate for deubiquitinating enzymes

Ist Teil von

• Tetrahedron, 2016-07, Vol.72 (27-28), p.4085-4090

Ort / Verlag

Elsevier Ltd

Erscheinungsjahr

2016

Quelle

ScienceDirect

Beschreibungen/Notizen

• A new fluorogenic substrate Ub-ACC (ubiquitin C-terminal 7-amino-4-carbamoylmethyl-coumarin) was developed for DUB (deubiquitinating enzyme) activity assays. This substrate can be synthesized with higher efficiency than the classical DUB substrate, Ub-AMC (ubiquitin C-terminal 7-amino-4-methylcoumarin). DUB assays using UCH-L3, OTUD2 and USP30 demonstrated that Ub-ACC shows nearly 2-fold higher sensitivity than Ub-AMC. [Display omitted]