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BibTeX
A novel method for monitoring troponin T fragment from rabbit skeletal muscle during aging using quartz crystal microbalance
Journal of the science of food and agriculture, 2016-08, Vol.96 (11), p.3944-3949
Iwasaki, Tomohito
Taniguchi, Hironobu
Hasegawa, Yasuhiro
Maeda, Naoyuki
Yamamoto, Katsuhiro
2016
Details
Autor(en) / Beteiligte
Iwasaki, Tomohito
Taniguchi, Hironobu
Hasegawa, Yasuhiro
Maeda, Naoyuki
Yamamoto, Katsuhiro
Titel
A novel method for monitoring troponin T fragment from rabbit skeletal muscle during aging using quartz crystal microbalance
Ist Teil von
Journal of the science of food and agriculture, 2016-08, Vol.96 (11), p.3944-3949
Ort / Verlag
Chichester, UK: John Wiley & Sons, Ltd
Erscheinungsjahr
2016
Link zum Volltext
Quelle
Wiley Online Library - AutoHoldings Journals
Beschreibungen/Notizen
BACKGROUND Troponin T (TnT) is degraded during aging of meat. The proteolytic fragment of TnT, especially the 30 kDa fragment, is used as one of indices for estimating aging of meat. We have tried to use quartz crystal microbalance (QCM), which is widely used to analyze interaction among macromolecules, to detect proteolytic fragments of TnT during aging of meat. RESULT The frequency of the QCM sensor with immobilized anti‐TnT antibody in high‐salt solution extracts of both myofibrils and whole meat decreased with time of aging. The staining intensity of the bands, including a 30 kDa fragment bound to anti‐TnT antibody, also increased with time of aging in western blotting. These results confirm that TnT is degraded during aging and released from thin filaments, and QCM analysis is sufficiently sensitive to detect the TnT fragments. CONCLUSION The QCM analysis of muscle and myofibrillar extracts using anti‐TnT antibody‐immobilized sensor can be used as a convenient tool for monitoring the extent of aging of meat. © 2015 Society of Chemical Industry
Sprache
Englisch
Identifikatoren
ISSN: 0022-5142
eISSN: 1097-0010
DOI: 10.1002/jsfa.7558
Titel-ID: cdi_proquest_miscellaneous_1825524956
Format
–
Schlagworte
Animals
,
Antibodies
,
Blotting, Western
,
Electrophoresis, Polyacrylamide Gel
,
Food Quality
,
Food science
,
Food Storage
,
Fragmentation
,
Immunoprecipitation
,
Japan
,
Meat
,
Meat - analysis
,
meat aging
,
Microbalances
,
Molecular Weight
,
Monitoring
,
Muscle, Skeletal - enzymology
,
Muscle, Skeletal - metabolism
,
Muscles
,
Myofibrils - enzymology
,
Myofibrils - metabolism
,
Peptide Fragments - analysis
,
Peptide Fragments - chemistry
,
Peptide Fragments - metabolism
,
Peptide Mapping
,
Proteolysis
,
quartz crystal microbalance
,
Quartz Crystal Microbalance Techniques
,
Quartz crystals
,
Rabbits
,
TNT
,
troponin T
,
Troponin T - analysis
,
Troponin T - chemistry
,
Troponin T - metabolism
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