Details

Autor(en) / Beteiligte

• Il'ina, A.V.
• Kurek, D.V.
• Zubareva, A.A.
• Il'in, M.M.
• Mestechkina, N.M.
• Varlamov, V.P.

Titel

Preparation and characterization of biopolymer nanoparticles based on lactoferrin-polysaccharide complexes

Ist Teil von

• Reactive & functional polymers, 2016-05, Vol.102, p.33-38

Ort / Verlag

Elsevier B.V

Erscheinungsjahr

2016

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• Optimal weight ratios for the preparation of lactoferrin (LF), N-succinyl chitosan (SCh) and galactomannan (GM)-based polyelectrolyte complexes (PEC) were determined. Soluble PEC were obtained using solutions of biopolymers: lactoferrin (2mg/ml), N-succinyl chitosan (8mg/ml) and galactomannan (16mg/ml) in 0.15M NaCl, pH6.5±0.1, under normal conditions at a weight ratio of 1:1 1:2 and 1:3 LF:GM; 1:1, 1:2, 1:3 and 1:4 LF:SCh; 1:3:2 and 1:2:3 LF:SCh:GM. Using the method of heat treatment below the temperature of protein denaturation from the soluble complexes were formed stable biopolymer particles which have size characteristics of 250–400nm, regular spherical shape, a negative zeta potential of 12–17mV and good storage stability. The load of lactoferrin in the particles was 76–87%. Yield of freeze dried particles was 70–80%. The obtained particles could eventually be used for the targeted transport of biologically active substances.