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In vitro antimicrobial susceptibility of Helcococcus kunzii and molecular analysis of macrolide and tetracycline resistance
Ist Teil von
European journal of clinical microbiology & infectious diseases, 2015-10, Vol.34 (10), p.2057-2061
Ort / Verlag
Berlin/Heidelberg: Springer Berlin Heidelberg
Erscheinungsjahr
2015
Quelle
MEDLINE
Beschreibungen/Notizen
Thanks to the recent advent of matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) technology,
Helcococcus kunzii
is now easily identifiable and considered as an opportunistic pathogen. However, data about antimicrobial susceptibilities remain very limited. The aim of the study was, then, to assess its in vitro susceptibility to 18 antimicrobial agents and to investigate the genetic basis of macrolide and tetracycline resistance. Thirty-nine human clinical isolates of
H. kunzii
collected from 2008 to 2013 were studied, as well as the type strain ATCC 51366
T
. Minimum inhibitory concentrations (MICs) of penicillin G, amoxicillin, cefotaxime, imipenem, gentamicin, erythromycin, clindamycin, quinupristin–dalfopristin, ciprofloxacin, levofloxacin, tetracycline, tigecycline, vancomycin, teicoplanin, linezolid, daptomycin, cotrimoxazole and rifampin were determined by the microdilution method. Screening for macrolide [
erm
(A) including
erm
(TR),
erm
(B),
erm
(C),
erm
(F),
erm
(T),
erm
(X),
msr
(A) and
mef
(A)] and tetracycline [
tet
(L),
tet
(M) and
tet
(O)] resistance genes was performed, as well as the detection of mutations in 23S rRNA. Except for one strain resistant to cefotaxime, all strains were categorised as susceptible to β-lactams, glycopeptides, linezolid, daptomycin and tigecycline. Whereas ciprofloxacin and gentamicin exhibited limited activity, 95 % of strains were categorised as susceptible to levofloxacin. Concerning erythromycin, a bimodal distribution was observed, with 29 ‘wild-type’ strains (MICs from 0.25 to 2 mg/L) and 11 ‘resistant’ strains (MICs ≥256 mg/L), including ten harbouring
erm
(TR). Two isolates exhibited acquired tetracycline resistance (MICs of 16 mg/L) by the production of
tet
(M). This large study on the in vitro antimicrobial susceptibility of
H. kunzii
suggests that β-lactams (especially penicillins) should be preferred for the treatment.