Details

Autor(en) / Beteiligte

• Ruiz de Alegría-Puig, C
• Aguirre-Quiñonero, A
• Agüero-Balbín, J
• Roiz-Mesones, M P
• Martínez-Martínez, L

Titel

Correlation between MALDI-TOF Vitek-MSTM system and conventional identification methods of gastrointestinal infection causing bacteria

Ist Teil von

• Revista española de quimioterapia, 2016-10, Vol.29 (5), p.265-268

Ort / Verlag

Spain

Erscheinungsjahr

2016

Quelle

MEDLINE

Beschreibungen/Notizen

• Rapid identification of pathogens is essential for the diagnosis of gastrointestinal infections. Matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry has shown to be effective and fast for the identification of microorganisms. The objective of this study was to evaluate the correlation between Vitek-MSTM and conventional methods for bacterial identification causing gastrointestinal infection. A total of 329 gastrointestinal pathogens were identified using Vitek-MSTM (v2 SARAMIS MS -ID, bioMérieux, Marcy-I´Étoile, France) and routine diagnostic methods simultaneously. In cases of discrepancy 16SrRNA gene sequencing was performed. The correlation between Vitek-MSTM and diagnostic methods was 100% except for Yersinia enterocolitica (94.1%), Helicobacter pylori (10%) and Aeromonas veronii (0 %). Vitek-MSTM is a quick and useful method for identification of enterophatogenic bacteria. It is necessary to improve the performance of the system for the identification of H. pylori and A. veronii.