Insecticidal and neural activities of candidate photoaffinity probes for neonicotinoid binding sites
2001
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- Autor(en) / Beteiligte
- Titel
- Insecticidal and neural activities of candidate photoaffinity probes for neonicotinoid binding sites
- Ist Teil von
- Bioscience, biotechnology, and biochemistry, 2001-07, Vol.65 (7), p.1534-1541
- Ort / Verlag
- Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry
- Erscheinungsjahr
- 2001
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Photoreactive derivatives of imidacloprid and its nitromethylene analogue were synthesized as candidate photoaffinity probes for identifying the amino acid residues of nicotinic acetylcholine receptors (nAChRs) that interact with the neonicotinoid insecticides. When the candidate probes were injected into American cockroaches, the nerve cord neural activity initially increased, then ceased and death of the insect followed. Both the nerve cord and toxicity were enhanced by changing the photoreactive substituent from the para position to the meta position on the spacer benzyl moiety. When tested on a Drosophila SAD / chicken beta2 hybrid, recombinant nAChR expressed in X/ENopus oocytes, the nitromethylene candidate probes showed agonist activity similar to that previously observed for imidacloprid.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0916-8451eISSN: 1347-6947DOI: 10.1271/bbb.65.1534Titel-ID: cdi_proquest_miscellaneous_18206012
- Format
- –
- Schlagworte
- ACETYLCHOLINE, Anabasine - metabolism, Animals, Binding Sites, Biological and medical sciences, Chemical control, Control, Drosophila, Female, Fundamental and applied biological sciences. Psychology, imidacloprid, In Vitro Techniques, INSECTICIDES, Insecticides - chemical synthesis, Insecticides - chemistry, Insecticides - pharmacology, neonicotinoid, NERVOUS SYSTEM, Nervous System - drug effects, Nervous System - metabolism, nicotinic acetylcholine receptor, nitromethylene, Oocytes - metabolism, Periplaneta, Photoaffinity Labels - chemical synthesis, Photoaffinity Labels - chemistry, Photoaffinity Labels - pharmacology, photoaffinity probe, Phytopathology. Animal pests. Plant and forest protection, Protozoa. Invertebrates, Receptors, Nicotinic - drug effects, Receptors, Nicotinic - genetics, Receptors, Nicotinic - metabolism, trifluoromethyldiazirine, Xenopus laevis