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•Pressurized liquid extraction (PLE) was utilized to extract fucoidan from brown seaweed.•High yield of fucoidan was obtained from PLE, when compared with the conventional method.•The structural characterization of crude polysaccharide confirms the presence of fucoidan.•Molecular weights of the extracted fucoidan were between 83.39–183.32kDa.•The obtained crude fucoidan showed properties of high antioxidant and emulsifying.
Pressurized liquid extraction (PLE) was utilized to extract sulfated polysaccharides (fucoidan) from brown seaweed Saccharina japonica. Various conditions of temperature (80–200°C), pressure (5–100bar), and solvents (water, 0.1% sodium hydroxide, 0.1% formic acid, 70% ethanol, 50% ethanol, and 25% ethanol) were assessed; the best crude fucoidan (CF) yield was 8.23%, obtained from 140°C and 50bar (sodium hydroxide). Compositional analysis, FT-IR, molecular weight, monosaccharides, TGA, UV–vis, XRD, and elemental analysis confirm that extracted polysaccharides revealed the features of fucoidan. Fucose was the main monosaccharide present in CF obtained by various solvent systems. All CF showed antioxidant activities as measured by DPPH radical and ABTS+ radical scavenging. CF demonstrates good emulsion-stabilizing capacities, especially with vegetable oils. This study demonstrates that PLE is an efficacious method for enhancing the yield of polysaccharides from S. japonica and that it could be a potential source of natural antioxidants and emulsifiers.