Details

Autor(en) / Beteiligte

• Sahoo, P.K.
• Swaminathan, T. Raja
• Abraham, Thangapalam Jawahar
• Kumar, Raj
• Pattanayak, S.
• Mohapatra, A.
• Rath, S.S.
• Patra, Avijit
• Adikesavalu, Harresh
• Sood, Neeraj
• Pradhan, P.K.
• Das, B.K.
• Jayasankar, P.
• Jena, J.K.

Titel

Detection of goldfish haematopoietic necrosis herpes virus (Cyprinid herpesvirus-2) with multi-drug resistant Aeromonas hydrophila infection in goldfish: First evidence of any viral disease outbreak in ornamental freshwater aquaculture farms in India

Ist Teil von

• Acta tropica, 2016-09, Vol.161, p.8-17

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2016

Quelle

MEDLINE

Beschreibungen/Notizen

• Detection of CyHV-2 in goldfish from Serampore, West Bengal, India and confirmation of infection based on PM findings, histology, TEM, CPE in CCKF cell lines and PCR assays. [Display omitted] •CyHV-2 was first detected with MDR Aeromonas hydrophila in goldfish in India.•CyHV-2 presence was confirmed by PCR, observed CPE and challenge study.•Experimental infection trials failed to reproduce disease in co-cultured rohu and koi carp. This outbreak report details of a mortality event where Cyprinid herpes virus-2 (CyHV-2) was detected in association with multidrug-resistant Aeromonas hydrophila infection in goldfish, Carassius auratus, from commercial farms. The goldfish exhibited large scale haemorrhages on the body, fins and gills, lepidorthosis, necrosed gills, protruded anus and shrunken eyes. White nodular necrotic foci in spleen and kidneys were noticed, along with necrosis and fusion of gill lamellae. Transmission electron microscopy of affected tissues revealed the presence of mature virus particles. Involvement of CyHV-2 was confirmed by PCR, sequencing and observed cytopathic effect in koi carp fin cell line along with experimental infection study. A bacterium isolated from the internal organs of affected fish was found to be pathogenic Aeromonas hydrophila having resistance to more than 10 classes of antibiotics. We postulate that CyHV-2 was the primary etiological agent responsible for this outbreak with secondary infection by A. hydrophila. The experimental infection trials in Labeo rohita and koi carp by intraperitoneal challenge with CyHV-2 tissue homogenates failed to reproduce the disease in those co-cultured fish species. This is the first report of a viral disease outbreak in organised earthen ornamental fish farms in India and bears further investigation.