Neurochemical research, 2016-09, Vol.41 (9), p.2425-2432
2016
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- Autor(en) / Beteiligte
- Titel
- Curcumin Attenuated Bupivacaine-Induced Neurotoxicity in SH-SY5Y Cells Via Activation of the Akt Signaling Pathway
- Ist Teil von
- Neurochemical research, 2016-09, Vol.41 (9), p.2425-2432
- Ort / Verlag
- New York: Springer US
- Erscheinungsjahr
- 2016
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Bupivacaine is widely used for regional anesthesia, spinal anesthesia, and pain management. However, bupivacaine could cause neuronal injury. Curcumin, a low molecular weight polyphenol, has a variety of bioactivities and may exert neuroprotective effects against damage induced by some stimuli. In the present study, we tested whether curcumin could attenuate bupivacaine-induced neurotoxicity in SH-SY5Y cells. Cell injury was evaluated by examining cell viability, mitochondrial damage and apoptosis. We also investigated the levels of activation of the Akt signaling pathway and the effect of Akt inhibition by triciribine on cell injury following bupivacaine and curcumin treatment. Our findings showed that the bupivacaine treatment could induce neurotoxicity. Pretreatment of the SH-SY5Y cells with curcumin significantly attenuated bupivacaine-induced neurotoxicity. Interestingly, the curcumin treatment increased the levels of Akt phosphorylation. More significantly, the pharmacological inhibition of Akt abolished the cytoprotective effect of curcumin against bupivacaine-induced cell injury. Our data suggest that pretreating SH-SY5Y cells with curcumin provides a protective effect on bupivacaine-induced neuronal injury via activation of the Akt signaling pathway.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0364-3190eISSN: 1573-6903DOI: 10.1007/s11064-016-1955-4Titel-ID: cdi_proquest_miscellaneous_1811878213
- Format
- –
- Schlagworte
- Activation, AKT protein, Anesthesia, Apoptosis, Apoptosis - drug effects, Attenuation, Biochemistry, Biomedical and Life Sciences, Biomedicine, Bupivacaine, Bupivacaine - pharmacology, Cell Biology, Cell injury, Cell Line, Tumor, Cell Survival - drug effects, Curcumin, Curcumin - pharmacology, Damage assessment, Humans, Inhibition, Injuries, Kinases, Local anesthesia, Low molecular weights, Membrane Potential, Mitochondrial - drug effects, Mitochondria, Molecular weight, Neurochemistry, Neurology, Neurons - drug effects, Neurons - metabolism, Neuroprotection, Neuroprotective Agents - pharmacology, Neurosciences, Neurotoxicity, Neurotoxicity Syndromes - drug therapy, Neurotoxicity Syndromes - metabolism, Original Paper, Pain, Pharmacology, Phosphatidylinositol 3-Kinases - metabolism, Phosphorylation, Phosphorylation - drug effects, Proto-Oncogene Proteins c-akt - metabolism, Signal transduction, Signal Transduction - drug effects, Signaling