Details

Autor(en) / Beteiligte

• Serra, Vanda Vaz
• Teixeira, Raquel
• Andrade, Suzana M.
• Costa, Sílvia M.B.

Titel

Design of polyelectrolyte core-shells with DNA to control TMPyP binding

Ist Teil von

• Colloids and surfaces, B, Biointerfaces, 2016-10, Vol.146, p.127-135

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2016

Quelle

MEDLINE

Beschreibungen/Notizen

• [Display omitted] ⿢DNA adsorption changes TMPyP interaction onto PSS/PAH core-shells (CS).⿢TMPyP/DNA binding modes are controlled by porphyrin and polyanion adsorption order.⿢DNA adsorption onto polyelectrolyte CS increases significantly the TMPyP uptake. The interaction of DNA with 5,10,15,20-tetrakis(4-N-methylpyridiniumyl)porphyrin (TMPyP) in polyelectrolyte core-shells obtained via layer by layer adsorption of poly(sodium 4-styrenesulfonate), PSS, and poly(allylamine hydrochloride), PAH, polyelectrolytes was followed by steady state, time resolved fluorescence and by Fluorescence Lifetime Imaging Microscopy (FLIM). Our results show that DNA adsorption onto polyelectrolyte core-shell changes the TMPyP interaction within PSS/PAH core-shells structure and increase significantly the TMPyP uptake. Specific DNA/TMPyP interactions are also altered by DNA adsorption favouring porphyrin intercalation onto GC pair rich regions. Circular dichroism (CD) spectra reveal that DNA undergoes important conformational changes upon adsorption onto the core-shell surface, which are reverted upon TMPyP encapsulation.