Details

Autor(en) / Beteiligte

• Gomes, Muller D
• Dao, Phuong
• Jeong, Keunhong
• Slack, Clancy C
• Vassiliou, Christophoros C
• Finbloom, Joel A
• Francis, Matthew B
• Wemmer, David E
• Pines, Alexander

Titel

(129)Xe NMR Relaxation-Based Macromolecular Sensing

Ist Teil von

• Journal of the American Chemical Society, 2016-08, Vol.138 (31), p.9747-9750

Ort / Verlag

United States

Erscheinungsjahr

2016

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• We report a (129)Xe NMR relaxation-based sensing approach that exploits changes in the bulk xenon relaxation rate induced by slowed tumbling of a cryptophane-based sensor upon target binding. The amplification afforded by detection of the bulk dissolved xenon allows sensitive detection of targets. The sensor comprises a xenon-binding cryptophane cage, a target interaction element, and a metal chelating agent. Xenon associated with the target-bound cryptophane cage is rapidly relaxed and then detected after exchange with the bulk. Here we show that large macromolecular targets increase the rotational correlation time of xenon, increasing its relaxation rate. Upon binding of a biotin-containing sensor to avidin at 1.5 μM concentration, the free xenon T2 is reduced by a factor of 4.