Details

Autor(en) / Beteiligte

• Dai, Shenglan
• Zhuo, Meng
• Song, Linlin
• Chen, Xiaohua
• Yu, Yongsheng
• Tang, Zhenghao
• Zang, Guoqing

Titel

Dendritic cell-based vaccination with lentiviral vectors encoding ubiquitinated hepatitis B core antigen enhances hepatitis B virus-specific immune responses in vivo

Ist Teil von

• Acta biochimica et biophysica Sinica, 2015-11, Vol.47 (11), p.870-879

Ort / Verlag

China

Erscheinungsjahr

2015

Quelle

MEDLINE

Beschreibungen/Notizen

• The activity of hepatitis B virus （HBV）-specific cytotoxic T lymphocytes （CTLs） plays a predominant role in the clearance of HBV. Dendritic cells （DCs） are key antigen-presenting cells and play an import- ant role in the initiation of immune responses. We previously verified that lentiviral vector encoding ubiquitinated hepatitis B core antigen （LV-Ub-HBcAg） effectively transduced DCs to induce matur- ation, and the mature DCs efficiently induced T cell polarization to Thl and generated HBcAg-specific CTLs ex vivo. In this study, HBV-specific immune responses of LV-Ub-HBcAg in BALB/c mice （H-2Kd） were evaluated. It was shown that direct injection of LV-Ub-HBcAg increased the production of cyto- kines IL-2 and IFN-y, elicited strong antibody responses, and remarkably generated a high percent- age of IFN-y＋CD8＋ T cells with HBV-specific CTL responses in BALB/c mice. In addition, direct injection of LV-Ub-HBcAg induced potent anti-HBV immune responses, similar to those elicited by in vitro-transduced DCs. In conclusion, the DC-based therapeutic vaccine LV-Ub-HBcAg elicited spe- cific antibody immune responses and induced robust specific CTL activity in vivo.