Details

Autor(en) / Beteiligte

• Sokolov, O. I.
• Selivanov, N. Y.
• Bogatyrev, V. A.
• Selivanova, O. G.
• Velikorodnaya, Y. I.
• Pocheptsov, A. Y.
• Filatov, B. N.
• Shchyogolev, S. Y.
• Dykman, L. A.

Titel

Synthesis and study on activity in vitro of the high purity human butyrylcholinesterase conjugated with gold nanoparticles

Ist Teil von

• Doklady. Biochemistry and biophysics, 2016-05, Vol.468 (1), p.232-234

Ort / Verlag

Moscow: Pleiades Publishing

Erscheinungsjahr

2016

Quelle

MEDLINE

Beschreibungen/Notizen

• The aim of this research was to design a method of immobilization of high-purity human butyrylcholinesterase on the surface of gold nanoparticles preserving the activity of the enzyme. In order to achieve this aim, the method of fractionation and purification of human butyrylcholinesterase from plasma was modified. The synthesis of 15-nm gold nanoparticles was carried out by citrated method. A method of conjugation of the high-purity butyrylcholinesterase with gold nanoparticles was developed. It was found that the Immobilization of butyrylcholinesterase on the surface of gold nanoparticles resulted in a significant (to 23%) increase in the specific activity of the enzyme.