Details

Autor(en) / Beteiligte

• Kim, Jeongtae
• Sunagawa, Masanobu
• Kobayashi, Shiori
• Shin, Taekyun
• Takayama, Chitoshi

Titel

Developmental localization of calcitonin gene-related peptide in dorsal sensory axons and ventral motor neurons of mouse cervical spinal cord

Ist Teil von

• Neuroscience research, 2016-04, Vol.105, p.42-48

Ort / Verlag

Ireland: Elsevier Ireland Ltd

Erscheinungsjahr

2016

Link zum Volltext

Quelle

Elsevier ScienceDirect Journals

Beschreibungen/Notizen

• •CGRP immunolabeling (IL) was first detected in ventral motor neurons on E13.•CGRP IL was localized in dorsal sensory fibers on E14.•CGRP-positive sensory fibers increased in chronological and sequential order.•Intensity of CGRP-IL increased during embryonic development.•Intensity of CGRP-IL markedly decreased in ventral motor neurons after birth. Calcitonin gene-related peptide (CGRP) is a 37-amino-acid neuropeptide, synthesized by alternative splicing of calcitonin gene mRNA. CGRP is characteristically distributed in the nervous system, and its function varies depending on where it is expressed. To reveal developmental formation of the CGRP network and its function in neuronal maturation, we examined the immunohistochemical localization of CGRP in the developing mouse cervical spinal cord and dorsal root ganglion. CGRP immunolabeling (IL) was first detected in motor neurons on E13, and in ascending axons of the posterior funiculus and DRG neurons on E14. CGRP-positive sensory axon fibers entered Laminae I and II on E16, and Laminae I through IV on E18. The intensity of the CGRP-IL gradually increased in both ventral and dorsal horns during embryonic development, but markedly decreased in the ventral horn after birth. These results suggest that CGRP is expressed several days after neuronal settling and entry of sensory fibers, and that the CGRP network is formed in chronological and sequential order. Furthermore, because CGRP is markedly expressed in motor neurons when axons are vastly extending and innervating targets, CGRP may also be involved in axonal elongation and synapse formation during normal development.